The present study aimed to determine the optimal dosage and fermentation time of Mirasolia diversifolia (leaves and stems) using Lactobacillus bulgaricus bacteria by observing the reduction in phytic acid, the activity of enzymes, pH of fermentation, number of bacterial colonies, and tannin concentration. An experiment was conducted using a completely randomized design (CRD) and 2x3 factorial design with three replications. The treatment factors were Factor A (L. bulgaricus dosages): A1 = 2% (g/v), A2 = 3% (g/v); and Factor B (fermentation times): B1 = 1 d, B2 = 3 d, B3 = 5 d. The dosage of L. bulgaricus and fermentation time had an interaction on phytic acid and its degradation, enzyme activities, pH fermentation, and the number of bacterial colonies. Tannin concentration was not affected. Mirasolia diversifolia fermented with 3% of L. bulgaricus for 5 d resulted in the lowest phytic acid content. Future research requires evaluating in vitro and in vivo dietary formulations for cattle using M. diversifolia fermented with L. bulgaricus.

ABSTRACT Background: Hepatitis B virus (HBV) infection and diabetes mellitus are major health problems associated with significant morbidity and mortality. The published literature suggests an association of diabetes mellitus with liver disease. However, the role of HBV infection in diabetes aetiology is still controversial. The present study was conducted to explore the veracity of this enigmatic association among Pakistani subjects. Methodology: The blood samples and clinical information were collected from chronic HBV-positive patients Group 1 (n = 120), and their age and gender were matched with those of the healthy control subjects Group 2 (n = 120). Hepatitis B virus-positive patients were also subdivided into two groups; (Group 1a and Group 1b) with and without liver cirrhosis for evaluation of the prevalence of diabetes. Results: The study revealed that there were statistically significant differences in the biochemical parameters in the HBV-positive and control groups. There was no correlation between diabetes and HBV with the prevalence of diabetes mellitus being similar in subjects with and without HBsAg (11.7% in the positive group and 10% in the controls). Since there were a relatively large number (32.5%) of HBV-positive patients with liver cirrhosis, a comparison of biochemical parameters was also carried out to evaluate the extent of the liver damage and its association with diabetes. During the comparison of HBV patients with and without cirrhosis for the prevalence of diabetes, no aetiologic association was found with diabetes. Conclusion: Study revealed that there was no correlation between HBV infection and diaabetes despite the significantly different biochemical parameters in the HBV-infected group and control subjects.

RESUMEN Antecedentes: La infección por el virus de la hepatitis B (VHB) y la diabetes mellitus son problemas de salud importantes asociados con morbilidad y mortalidad significativas. La literatura publicada sugiere una asociación de la diabetes mellitus con las enfermedades hepáticas. Sin embargo, el papel de la infección por VHB en la etiología de diabetes sigue siendo contro-versial. El presente estudio fue conducido con el propósito de explorar la veracidad de esta enigmática asociación entre sujetos paquistaníes. Metodología: Se recogieron muestras de sangre e información clínica de pacientes crónicos VHB positivos Grupo 1 (n = 120), y su edad y género fueron comparados con los de los sujetos sanos del control Grupo 2 (n = 120). Los pacientes positivos al virus de la hepatitis B también se subdividieron en dos grupos, a saber, (Grupo 1a y Grupo 1b) con y sin cirrosis hepática en relación con la prevalencia de la diabetes. Resultados: El estudio reveló que hubo diferencias significativas en estos dos grupos en los parámetros bioquímicos entre el grupo de control y el grupo VHB positivo. En estos dos grupos no hubo correlación entre la diabetes y el VHB. Puesto que hubo un número relativamente grande (32.5%) de pacientes VHB positivos con cirrosis hepática, se realizó también una comparación de los parámetros bioquímicos a fin de comprender el grado del daño hepático y su asociación con la diabetes. Durante la comparación de los pacientes con VHB con y sin cirrosis en relación con la prevalencia de diabetes, no se halló asociación etiológica con la diabetes. Conclusión: Este estudio reveló que no hubo correlación entre la infección por VHB y la diabetes, a pesar de los parámetros bioquímicos significativamente diferentes entre el grupo infectado por el VHB y los sujetos del control.

RESUMO: Um experimento foi conduzido para avaliar os efeitos resultantes do sistema de pré-germinação, priming, padrões de plantio e tratamentos de controle de ervas daninhas sobre o espectro da erva daninha, rendimento de grãos e rentabilidade do trigo, durante o período de desenvolvimento da planta de 2013-14. As sementes foram preparadas de duas formas: sem a utilização do sistema de priming, e sob condicionamento osmótico, osmopriming. Os padrões de plantio foram semeadura a lance (broadcast) e em linha (row plantation) e quarto dosagens de herbicidas foram aplicadas (pyroxulam nas dosagens 14,08, 10,56, 7,04 e 7,521 g i.a. ha-1), em um delineamento inteiramente casualizado em sistema de parcela subdividida split-plot. Os dados relativos à densidade de plantas daninhas, ao estabelecimento, ao crescimento e ao rendimento dos traços de trigo associados foram registrados e analisados por técnica de análise de variância usando software estatístico DSAASTAT. Os resultados revelaram que a contagem final de emergência foi significativamente melhorada pela semeadura em linha. Os tratamentos de preparação de sementes resultaram em emergência prévia de colheita e propagação do dossel e também diminuiu a densidade de ervas daninhas e o peso seco em 13% e 18%, respectivamente, a 60 DAS (dias após a semeadura). Além disso, o rendimento de grãos foi melhorado em 8% também nas parcelas de tratamento de cebola. A semeadura em linha do tratamento do trigo diminuiu a densidade de ervas daninhas e o peso seco em 17% e 25%, respectivamente, com um rendimento de grão melhorado em 14% em relação ao método de semeadura a lance. O Pyroxulam com uma taxa de 14,08 g i.a. ha-1 aplicado a 60 DAS reduziu a densidade total de ervas daninhas e o peso seco em 88% e 96%, respectivamente, e o rendimento de grãos foi melhorado em 36% em relação às parcelas de verificação de plantas daninhas. Em conclusão, as sementes sob condicionamento osmótico (1% de KCl) semeadas em linhas de 22,5 cm de distância proporcionaram maior produção de trigo, as ervas daninhas foram devidamente controladas por aplicação de piroxulam aplicado na dose recomendada (14,08 g i.a. ha-1) e também 75% da dose recomendada sob condições de Faisalabad.

ABSTRACT: An experiment was conducted to evaluate the effect of priming, planting patterns and weed control treatments on weed spectrum, grain yield and profitability of wheat, during the growing season 2013-14. Two levels of seed priming (no priming, osmopriming) and planting patterns (broadcast, row plantation), and four levels of herbicide (pyroxulam at the rate of 14.08, 10.56, 7.04 and 7.521 g a.i. ha-1) were employed in the experiment, laid out in a randomized complete block design with split-split plot arrangement. The data regarding weed density, stand establishment, growth, and yield associated traits of wheat were recorded and analyzed by analysis of variance technique using statistical software DSAASTAT. The results revealed that the final emergence count was improved significantly by line sowing. The seed priming treatments resulted in early crop emergence and canopy spread, and it also decreased weed density and dry weight by 13% and 18%, respectively at 60 DAS (days after sowing). Moreover, grain yield was improved by 8% in priming treatment plots. Whereas, line sowing of wheat treatment decreased the weed density and dry weight by 17% and 25% respectively, with improved grain yield by 14% over the broadcast method. Pyroxulam at the rate of 14.08 g a.i. ha-1 applied at 60 DAS reduced total weed density and dry weight by 88% and 96% respectively and grain yield was improved by 36% over weedy check plots. In conclusion, osmoprimed seeds (1% KCl) sown in lines 22.5 cm apart gave higher wheat yields where weeds were properly controlled through application of pyroxulam applied at its recommended dose (14.08 g a.i. ha-1) and also 75% of recommended dose under Faisalabad conditions.